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ATF6 Antibody
SKU: orb1239224
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.The immunogen is located within amino acids 30 - 80 of ATF6. |
| Target | ATF6 |
| Purification | ATF6 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | ATF6 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha
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KO Validation of ATF6, Loading: 15 µg of human HeLa cell lysate Antibodies ATF6 orb1239224, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Human Cell Lines, Loading: 15 µg of human cell lysate Antibodies ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.
WB Validation in Mouse Tissue, Loading: 15 µg of mouse tissue lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Rat Heart, Loading: 15 µg of rat kidney lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of ATF6 in Human Testis Tissue, Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ATF6 in Mouse Kidney Tissue, Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ATF6 in Rat Kidney Tissue, Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ATF6 Antibody (orb1239224)
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