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Bcl-2 Mouse Monoclonal Antibody
SKU: orb499610
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB=1:1000-5000, ICC/IF=1:100-500, Flow-Cyt=1μg/Test |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 1A5 |
| Immunogen | Recombinant human Bcl-2 protein (1-211/239 (C-6x His-Tag)aa) |
| Target | BCL2 |
| Molecular Weight | 26 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
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Sample: Recombinant human Bcl-2 protein Lysate at 40 ug, Primary: Anti-Bcl-2 (orb499610) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26kD, Observed band size: 26kD.
Sample: U937 (human) cell Lysate at 40 ug, Primary: Anti-Bcl-2 (orb499610) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26kD, Observed band size: 26kD.
Quick Database Links
Gene Symbol
BCL2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
Bcl-2 Mouse Monoclonal Antibody (orb499610)
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