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CD3e Antibody
SKU: orb606527
Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FACS, WB |
|---|---|
| Dilution Range | Flow cytometry: 1-2ug/10^6 cells,ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | C3e/2478 |
| Immunogen | A portion of amino acids 23-119 were used as the immunogen for the CD3e antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Flow cytometry testing of human Jurkat cells with CD3e antibody (clone C3e/2478); Red = isotype control, Blue = CD3e antibody.
Western blot testing of human Jurkat cells with CD3e antibody (clone C3e/2478). Expected molecular weight ~23 kDa.
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD3e antibody (clone C3e/2478). These results demonstrate the foremost specificity of the C3e/2478 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone C3e/2478) as confirmation of integrity and purity.
Quick Database Links
UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD3e Antibody (orb606527)
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