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CD3e Antibody
SKU: orb750028
Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 4
| Tested Applications | FACS, WB |
|---|---|
| Dilution Range | Western blot: 1-2ug/ml for 60 min at RT,Flow cytometry: 1-2ug/10^6 cells |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b, kappa |
| Clone No. | C3e/1308 |
| Immunogen | Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_1.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_2.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_3.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_4.png)
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Western blot testing of human Jurkat cell lysate with CD3e antibody (clone C3e/1308). Predicted molecular weight ~23 kDa.
Western blot testing of 1) a partial recombinant protein and 2) human Jurkat cell lysate with CD3e antibody (clone C3e/1308). Predicted molecular weight ~23 kDa.
SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone C3e/1308). Confirmation of Integrity and Purity of the Antibody.
Flow cytometry testing of human Jurkat cells with CD3e antibody (clone C3e/1308); Red = isotype control, Blue = CD3e antibody.
Quick Database Links
UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
CD3e Antibody (orb750028)
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