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CD3e Antibody
SKU: orb2640872
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FACS, IHC-P, WB |
|---|---|
| Dilution range | ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | C3e/1931 |
| Immunogen | A recombinant human partial protein corresponding to amino acids 23-119 from the human CD3 epsilon chain were used as the immunogen for the CD3e antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Flow cytometry testing of human Jurkat cells with CD3e antibody (clone C3e/1931); Red = isotype control, Blue = CD3e antibody.
IHC testing of human spleen tissue with CD3e antibody (clone C3e/1931). Required HIER: boil tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min.
SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone C3e/1931) as confirmation of integrity and purity.
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD3e antibody (clone C3e/1931). These results demonstrate the foremost specificity of the C3e/1931 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Western blot testing of human Jurkat cell lysate with CD3e antibody (clone C3e/1931).
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD3e Antibody (orb2640872)
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