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CD3E Antibody

SKU: orb1248116

Description

CD3E Antibody

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityPorcine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 22kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kDa according to NP_000724.1). Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-RKGQRDLYSGLNQR
TargetCD3E
Molecular WeightApprox 22kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain

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CD3E Antibody

orb1248116 (0.1 ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CD3E Antibody (orb1248116)

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0.1 mg
¥ 11,440.00
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