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CD3E Antibody
SKU: orb1252166
Description
Research Area
Cancer Biology, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Bovine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | PC3/188A |
| Immunogen | Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody. |
| Target | CD3E |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Concentration | 0.2 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
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![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_1.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_2.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_3.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_4.png)
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Western blot testing of human Jurkat cell lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.
SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation of Integrity and Purity of the Antibody.
Quick Database Links
Gene Symbol
CD3E
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
CD3E Antibody (orb1252166)
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