CD40 Recombinant Protein

SKU: orb1228161

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Description

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	WB
Application Notes	This recombinant protein can be used for WB. For research use only.

Key Properties

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Source	HEK293 cells
Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized rhCD40-Fc at 2μg/ml (100μl/well) can bind biotinylated human CD40L with a linear range of 7. 8 - 125 ng/mL.
Target	CD40
Tag	C-Fc Tag
Molecular Weight	45.3 kDa
Protein Sequence	Glu 21 - Arg 193
Purity	>95% as determined by SDS-PAGE.

Storage & Handling

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Storage	Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Tris with Glycine, Arginine and NaCl, pH 7.5
Disclaimer	For research use only

Alternative Names

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CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50

Human CD40L [orb2994532]

Conjugation:	Unconjugated
Purity:	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
MW:	Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions

Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg

Human CD40L [orb2993921]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
MW:
Predicted: 42.7 KDa. Observed: 50&90 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg
Biotinylated Human CD40L [orb2993734]
Conjugation:
Biotin
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions
Sizes Available: 20 μg, 100 μg

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The purity of rh CD40 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

Quick Database Links

Gene Symbol

CD40

UniProt

P25942

RefSeq (Protein):NP_001241.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001241.1

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Protocol Information

Western Blot (IB, immunoblot)

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