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CD40 Recombinant Protein
Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | CHO cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA assay. |
| Target | CD40 |
| Tag | Fc Tag |
| Protein Sequence | The extracellular domain of human CD40 (aa 21-193) is fused to the N-terminus of the Fc region of human IgG1. |
| Purity | ≥98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
Storage & Handling
−| Storage | Stable for at least 1 year after receipt when stored at -20˚°C. Working aliquots are stable for up to 3 months when stored at -20˚°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human CD40L [orb2995248]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2994033]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.1 KDa. Observed: 19-22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2994532]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2993921]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 42.7 KDa. Observed: 50&90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgBiotinylated Human CD40L [orb2993734]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions
20 μg, 100 μg

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Protocol Information
CD40 Recombinant Protein (orb1230022)
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