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CD40 Recombinant Protein
Description
Research Area
Images & Validation
−
| Application Notes |
|---|
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Blocks the binding of CD40L to CD40. |
| Target | CD40 |
| Tag | Fc Tag |
| Protein Sequence | The Fc portion of human IgG1 is fused to the C-terminus of human CD40 (aa 21-193). |
| Purity | >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20˚°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 50 μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
| Concentration | 1mg/ml after reconstitution. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human CD40L [orb2995248]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2994033]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.1 KDa. Observed: 19-22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2994532]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2993921]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 42.7 KDa. Observed: 50&90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgBiotinylated Human CD40L [orb2993734]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions
20 μg, 100 μg

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Protocol Information
CD40 Recombinant Protein (orb1230141)
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