CD47 Antibody / IAP / Integrin Associated Protein

SKU: orb749334

Description

This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is an ~ 50kDa glycoprotein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Images & Validation

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Tested Applications	FACS, IF, WB
Dilution range	Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	B6H12.2
Immunogen	Intact CD47 purified from placenta was used as the immunogen for this antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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CD47 Antibody / IAP / Integrin Associated Protein

Flow cytometry testing of PFA-fixed human MCF-7 cells with CD47 antibody (clone B6H12.2); Red = isotype control, Blue = CD47 antibody.

Immunofluorescent staining of PFA-fixed human MCF-7 cells with CD47 antibody (green, clone B6H12.2) and Reddot nuclear stain (red).

Immunofluorescent staining of PFA-fixed human Jurkat cells with CD47 antibody (green, clone B6H12.2) and Reddot nuclear stain (red).

Flow cytometry testing of live human Jurkat cells with CD47 antibody (clone B6H12.2); Red = isotype control, Blue = CD47 antibody.

Immunofluorescent staining of live human Jurkat cells with CD47 antibody (green, clone B6H12.2).

Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35 kDa but observed at 35-60 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free CD47 antibody (clone B6H12.2) as confirmation of integrity and purity.

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Protocol Information

Western Blot (IB, immunoblot)

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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