Collagen IV Antibody

SKU: orb1537118

Description

Images & Validation

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Tested Applications	ELISA, IHC-P, WB
Dilution range	ELISA (1:100 - 1:200), IHC-P (1:100), WB (1:100 - 1:200)
Reactivity	Human
Application Notes	Further information: Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1,k
Immunogen	Human placenta.
Target	Collagen IV
Purification	Protein A/G purified

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Tris, pH 7.3-7.7, 1% rAlbumin, < 0.1% Sodium Azide
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

Collagen IV

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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