Collagen IV Antibody

SKU: orb1537863

Description

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, IP, WB
Dilution range	ELISA (1:5000 - 1:50000), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:1000 - 1:10000)
Reactivity	Bovine, Human, Mammal
Application Notes	Further information: Immunohistochemistry: LS-B2717 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2717 was determined to be 2.5 ug/ml. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Collagen Type IV from human and bovine placenta.
Target	Collagen IV
Purification	Affinity chromatography

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration	1 mg/ml
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

Collagen IV

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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