Collagen IV Antibody

SKU: orb1539266

Description

Images & Validation

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Tested Applications	ICC, IHC, IHC-Fr, IHC-P, WB
Dilution range	ICC, IHC, IHC-Fr (1:25 - 1:100), IHC-P (1:75 - 1:100), WB (1:100 - 1:1000)
Reactivity	Bovine, Human, Porcine, Rat
Application Notes	Further information: Methanol fixation recommended for immunocytochemistry. For immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Full length Type IV collagen isolated from human placenta
Target	Collagen IV
Purification	Antiserum

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Antiserum, 0.09% sodium azide. Antiserum, 0.09% sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

Collagen IV

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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ICC

Immunocytochemistry

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