Collagen IV Rabbit Polyclonal Antibody

SKU: orb101038

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Description

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain (1571-1669/1669aa)
Target	COL4A1
Molecular Weight	165 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Arresten; Canstatin; COL4A1; HANAC; ICH; POREN1; Collagen alpha 1(IV) Chain; Collagen IV alpha 1 Polypeptide; Collagen Of Basement Membrane alpha 1 Chain; Collagen Of Basement Membrane alpha 2 Chain; Collagen Type IV alpha 1; DKFZp686I14213; FLJ22259; collagen alpha-1(IV) chain preproprotein; collagen alpha-1(IV) chain preproprotein; Col4a1 protein; collagen of basement membrane, alpha-1 chain; collagen IV, alpha-1 polypeptide; collagen alpha-1(IV) chain; COL4A1 NC1 domain; CO4A1_HUMAN; Collagen Ⅳ; Collagen Type Ⅳ.

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Blank control: Hepg2 Cells (blue). Primary Antibody: Rabbit Anti-Collagen IV/AF647 Conjugated antibody, Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Sample: Lane 1: Recombinant human Collagen IV protein, N-His, Primary: Anti-Collagen IV (orb101038) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 165 kDa, Observed band size: 28 kDa.

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Collagen IV Polyclonal Antibody, Unconjugated (orb101038) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

COL4A1

UniProt

P02462

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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