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ENaC Alpha antibody
SKU: orb101489
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:100-800, IHC-P: 1:100-600, IF/ICC: 1:100-600 |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ENaC Alpha. Please contact us for the exact immunogen sequence. The peptide is available as orb12930. |
| Target | ENaC Alpha |
| Molecular Weight | 76 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−anti-Alpha ENaC 2 antibody, anti-Alpha ENaC antibody, anti-Alpha NaCH antibody, anti-Alpha-ENaC antibody, anti-Alpha-NaCH antibody, anti-Amiloride sensitive epithelial sodium channel alpha subunit antibody, anti-Amiloride sensitive sodium channel subunit alpha antibody, anti-ENaCa antibody, anti-ENaC alpha antibody, anti-Epithelial Na+ channel subunit alpha antibody, anti-FLJ21883 antibody, anti-Nonvoltage gated sodium channel 1 subunit alpha antibody, anti-SCNEA antibody, anti-SCNN 1 antibody, anti-SCNN1 antibody, anti-SCNN1A antibody, anti-Sodium channel nonvoltage gated 1 alpha. antibody
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IHC-P staining of rat pancreas tissue using ENaC Alpha antibody (2.5 ug/ml)
IF image of guinea pig kidney tissue using ENaC Alpha antibody (2.5 ug/ml)
IHC-P staining of rat lung tissue using anti-ENaC Alpha (2.5 ug/ml)
Western blot analysis of rat uterus (lane 1), mouse kidney (lane 2), rat lung (lane 3), rat pancreas (lane 4), rat liver (lane 5), rat brain (lane 6), rat vitreous (lane 7) using ENaC Alpha antibody (1 ug/ml)
IF analysis of guinea pig kidney tissue using ENaC Alpha antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded guinea pig kidney tissue using anti-ENaC Alpha (2.5 ug/ml)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
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Ye Gao 1, Fei Cao 2, Xinyi Tian 1, Qianping Zhang 3, Congcong Xu 1, Bowen Ji 1, Ye-An Zhang 1, Linan Du 1, Jun Han 1, Li Li 1, Siyu Zhou 1, Yuqiang Gong 1, Binyu Ying 1, Fang Gao-Smith 4, Shengwei Jin Inhibition the ubiquitination of ENaC and Na,K-ATPase with erythropoietin promotes alveolar fluid clearance in sepsis-induced acute respiratory distress syndrome Biomed Pharmacother, 174, 116447 (2024)
Applications
WB
Reactivity
Rat
MC Iachini, A Coglot, D Tace, N Elia, F Rusconi, F Cosentino, G Lopez, M Crosti, TD Usal… Generation of Human 3D Airway Assembloids for Advanced Modeling International Journal of Biological Sciences, (2025)
Han, Jun et al. Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury J. Cell. Mol. Med., 24, 4736-4747 (2020)
ENaC Alpha antibody (orb101489)
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