SCNN1A Rabbit Polyclonal Antibody

SKU: orb500994

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Description

Images & Validation

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Item 1 of 4

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Mouse
Predicted Reactivity	Human, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Alpha-ENaC (201-300/669aa)
Target	SCNN1A
Molecular Weight	76 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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SCNNA_HUMAN; Amiloride-sensitive sodium channel subunit alpha; alpha-NaCH; Epithelial Na(+) channel subunit alpha(alpha-ENaC; ENaCA); Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN1; sodium channel epithelial 1 subunit alpha; BESC2; ENaCa; LIDLS3; PHA1B1; ENaCalpha;

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25 ug total protein per lane of various lysates probed with SCNN1A polyclonal antibody, unconjugated (orb500994) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated (orb500994) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated (orb500994) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SCNN1A

UniProt

P37088

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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