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Fab Rat IgG (H&L) Antibody
SKU: orb348495
Description
Images & Validation
−Item 1 of 1
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:5,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG Fab |
| Immunogen | Rat IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Goat Fab Anti-Rat IgG Antibody, Goat Fab Fragment Anti-Rat IgG Antibody
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Dot Blot of Fab Goat Anti-Rat IgG Antibody. Rat IgG (p/n orb2652744) (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: Fab Goat Anti-Rat IgG at 1.0 µg/ml for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n orb347032) at 1:40000 for 30 mins at RT. Block: BlockOut buffer (p/n orb348644) for 30 mins at RT. Exposure: 5 sec.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Fab Rat IgG (H&L) Antibody (orb348495)
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