F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb348285

Description

F(ab')2 Mouse IgG (H&L) antibody (Peroxidase)

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:7,500 - 1:30,000, IHC: 1:500 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Mouse
Application Notes	Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Mouse IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Donkey F(ab')2 Anti-Mouse IgG Antibody peroxidase Conjugation, Donkey Fab2 Anti-Mouse IgG HRP Conjugated Antibody

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F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Intrinsic expression level of Hmga2 does not significantly affect tumor-prone MCSC-originating melanoma development and in vivo melanoma growth rate. (E) Western blotting was performed for control and Hmga2 gene-edited C.MM cell lines. Loading control, α-tubulin.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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