GDNF Antibody (HRP)

SKU: orb2652575

Description

Rabbit Anti-Human GDNF Polyclonal

Images & Validation

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Tested Applications	WB
Dilution range	WB (1:1000); optimal dilutions for assays should be determined by the user.
Reactivity	Human, Mouse, Rat
Application Notes	1 µg/mL of SPC-810 was sufficient for detection of GDNF in 15 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human GDNF Protein, Full length (AA78-211)
Target	GDNF
Purification	Protein A Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration	1mg/mL
Disclaimer	For research use only

Alternative Names

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GDNF, Glial cell line-derived neurotrophic factor, Glial Cell Line Derived Neurotrophic Factor, Glial cell derived neurotrophic factor, Glial derived neurotrophic factor, Astrocyte-derived trophic factor, Astrocyte derived trophic factor, Astrocyte derived trophic factor 1, ATF, ATF1, ATF2, Atf, HFB1 GDNF, hGDNF, HSCR3, GDNF_HUMAN

Quality Guarantee

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Western blot analysis with anti-GDNF polyclonal antibody showing detection of human recombinant GDNF protein. This recombinant protein has a predicted molecular weight of 15.2 kDa. Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:5000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 9.122 seconds.

Western blot analysis with anti-GDNF polyclonal antibody showing detection of GDNF protein in rat brain lysate (prepared in-house). Rat GNDF protein with signal and pro-peptide segments has a predicted molecular weight of 23.6 kDa (UniProt Q07731). Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 7.299 seconds. NOTE secondary antibody shows some non-specific binding in the control, but not to the GNDF protein.

Quick Database Links

Gene Symbol

GDNF

UniProt

P39905

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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