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GFAP Rabbit pAb
SKU: orb539616
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 350-432 of human GFAP (NP_002046.1). |
| Molecular Weight | 45-50kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ALXDRD, GFAP
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Immunofluorescence analysis of paraffin-embedded mouse brain using GFAP Rabbit pAb (orb539616) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of paraffin-embedded rat brain using GFAP Rabbit pAb (orb539616) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of U-251MG cells using GFAP Rabbit pAb (orb539616) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded mouse brain using GFAP Rabbit pAb (orb539616) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using GFAP Rabbit pAb (orb539616) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat cerebellum using GFAP Rabbit pAb (orb539616) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western blot analysis of various lysates using GFAP Rabbit pAb (orb539616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Filter by Applications
Filter by Species
Miriam Corraliza-Gomez et al. Bioreactor expansion of human neural progenitor cells for exosome scalable production and miRNA-engineering J Biol Eng, (2026)
Applications
ICC
Reactivity
Human
GFAP Rabbit pAb (orb539616)
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