GNAS Rabbit Polyclonal Antibody

SKU: orb2176

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Description

GNAS Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Human, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GNAS (701-800/1037aa)
Target	GNAS
Molecular Weight	120 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GNAS1; NESP55; SCG6; AHO; AIMAH1; C20orf45; GPSA; GSA; GSP; NESP; PITA3; POH; SgVI; 5530400H20Rik; A930027G11Rik; C130027O20Rik; Galphas; Gnasxl; Gs-alpha; Nespl; Oed-Sml; Oedsml; P1; P2; P3; PHP1A; PHP1B; PORGSA1; ALEX; G-alpha-8; Gnpas; GNAS2_BOVIN; GNAS; Adenylate cyclase-stimulating G alpha protein; 3.6.5.-; GNAS_CANLF; GNAS2_HUMAN; ALEX_HUMAN; Alternative gene product encoded by XL-exon; GNAS1_HUMAN; Extra large alphas protein (XLalphas); GNAS2_MOUSE; GNAS1_MOUSE; GNAS_PIG; GNAS2_RAT; GNAS1_RAT;

Quality Guarantee

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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (G protein alpha) Polyclonal Antibody, Unconjugated (orb2176) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-GNAS (orb2176) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 111 kDa, Observed band size: 120 kDa.

Quick Database Links

Gene Symbol

GNAS

UniProt

P63092

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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