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HSPB1 Antibody

SKU: orb1252760

Description

HSPB1 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsFC, IF, IHC, WB
ReactivityGallus, Human, Mouse, Primate, Rat, Sheep
Application Notes
Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLWB: 0.25-0.5 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone No.G3.1
ImmunogenPartially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
TargetHSPB1
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
DisclaimerFor research use only

Alternative Names

Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

HSPB1 Antibody (orb1252760)

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Available Sizes

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100 μg
¥ 14,820.00
DispatchUsually dispatched within 5-10 working days
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