HSP27 Mouse Monoclonal Antibody

SKU: orb500512

Description

Research Area

Actina, Cancer Research, Cardiovascular Research, Signal Transduction, Tumor Biomarkers

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution range	WB=1:1000-2000
Reactivity	Human

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	1G12
Immunogen	Recombinant human HSP27 Protein
Target	HSPB1
Molecular Weight	27 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Heat shock 27kDa protein; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; Estrogen-regulated 24 kDa protein; Heat shock 25kDa protein 1; Heat shock 25kDa protein 1; Heat shock 27 kDa protein; Heat shock 27kD protein 1; Heat shock 27kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat Shock Protein 27; Heat shock protein beta 1; Heat shock protein beta-1; Heat Shock Protein27; Heat Shock Protein27; HMN2B; HS.76067; Hsp 25; Hsp 25; Hsp 27; Hsp 27; Hsp 28; Hsp 28; Hsp B1; Hsp B1; Hsp25; Hsp25; HSP27; Hsp28; Hsp28; HspB1; HspB1; HSPB1_HUMAN; SRP27; Stress responsive protein 27; Stress-responsive protein 27.

Quality Guarantee

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Sample: Hela Cell (Human) Lysate at 40 ug, MCF-7 Cell (Human) Lysate at 40 ug, 293T Cell (Human) Lysate at 40 ug, A673 Cell (Human) Lysate at 40 ug, Primary: Anti-HSP27 (orb500512) at 1/2 000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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