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HSPB1 Antibody
SKU: orb1253724
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | HSP27 (Phospho-Ser78) antibody was raised against a peptide sequence around phosphorylation site of serine 78 (A-L-S (p) -R-Q) derived from Human HSP27. |
| Target | HSPB1 |
| Molecular Weight | 27 kDa |
| Purification | Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, KIAA0204, STK2, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25
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Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78).
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001531.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HSPB1 Antibody (orb1253724)
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