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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | ①Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14, the EC50 is 137.8-233.4 ng/ml. |
| Tag | C-terminal hFc1-Myc-tagged |
| Expression Region | 31-150aa |
| Protein Length | Partial |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
| MW | 43.9 kDa |
| Purity | Greater than 94% as determined by SDS-PAGE. |
| Protein Sequence | KESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTDVKSASERPSKDEMAS |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−(B- and T-lymphocyte-associated protein)(CD272)
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The protein has a predicted molecular mass of 41.3 kDa after removal of the signal peptide.
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Recombinant Human BTLA Protein [orb2994992]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.79 KDa. Observed: 30 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14, the EC50 is 137.8-233.4 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized Human BTLA at 2μg/ml can bind Anti-BTLA recombinant antibody, the EC50 is 1.763-3.542 ng/mL.
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Human BTLA protein (orb624122)
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