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Description
Research Area
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Source | E.coli |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CD40-Fc at 5μg/ml can bind Human CD40L, the ED50 of Human CD40L is 6.05 ng/ml. |
| Tag | Tag-Free |
| Expression Region | 113-261aa |
| Protein Length | Partial |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
| MW | 16.2 kDa |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Protein Sequence | MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Human CD40LG protein [orb705340]
Greater than 87% as determined by SDS-PAGE.
44.5 kDa
Mammalian cell
1 mg, 20 μg, 100 μgCD40L/Cd40lg Rabbit Polyclonal Antibody [orb402440]
ELISA, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
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100 μgRecombinant Human CD40L Protein [orb2995248]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human CD40L Protein [orb2993075]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.8 KDa. Observed: 19-24 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human CD40L Protein (Biotin) [orb2993734]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions
20 μg, 100 μg

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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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UniProt Details
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Protocol Information
Human CD40LG protein (orb594873)
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