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Human sCD40L Protein

SKU: orb428314

Description

Recombinant of human sCD40L protein

Images & Validation

Application Notes
Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.1 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of CD40 as a Reference Standard

Key Properties

SourceEscherichia Coli
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of IL-12 induction & IL-8 production by human PBMC was found to be 5-10 ng/ml.
PurityGreater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Storage & Handling

StorageStability: Lyophilized sCD40L although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD154 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Form/AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
Buffer/PreservativesLyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Human sCD40L Protein (orb428314)

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Available Sizes

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10 μg
¥ 3,250.00
50 μg
¥ 4,550.00
1 mg
¥ 25,610.00
DispatchUsually dispatched within 5-10 working days
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