Human TGF beta 1 protein

SKU: orb707059

Description

Recombinant Human TGF beta 1 Protein is produced by mammalian expression system and the target gene encoding Leu30-Ser390 is expressed

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Human
Protein Sequence	HHHHHHHHLS TSKTIDMELV KRKRIEAIRG QILSKLRLAS PPSQGEVPPG PLPEAVLALY NSTRDRVAGE SAEPEPEPEA DYYAKEVTRV LMVETHNEIY DKFKQSTHSI YMFFNTSELR EAVPEPVLLS RAELRLLRLK LKVEQHVELY QKYSNNSWRY LSNRLLAPSD SPEWLSFDVT GVVRQWLSRG GEIEGFRLSA HCSCDSRDNT LQVDINGFTT GRRGDLATIH GMNRPFLLLM ATPLERAQHL QSSRHRRALD TNYCFSSTEK NCCVRQLYID FRKDLGWKWI HEPKGYHANF CLGPCPYIWS LDTQYSKVLA LYNQHNPGAS AAPCCVPQAL EPLPIVYYVG RKPKVEQLSN MIVRSCKCS
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of 50mM Glycine, 50mM NaCl, pH 4.0.
Disclaimer	For research use only

Alternative Names

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TGFB; Transforming growth factor beta-1; TGF-beta-1

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Quick Database Links

UniProt

P01137

NCBI Gene ID

7040

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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