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IL-15 Recombinant Protein
Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | ED50 is less than 0.5 ng/ml as determined by the dose dependent stimulation of mouse CTLL-2 cell proliferation.Specific Activity of 2.0 x 106 IU/mg. |
| Target | IL15 |
| Tag | Tag Free |
| Molecular Weight | 12.5 kD |
| Protein Sequence | Asn49-Ser162 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL15 Protein, hFc Tag [orb1291067]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 38.9 kDa after removal of the signal peptide. The apparent molecular mass of IL15-hFc is approximately 35-55kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μgRecombinant Cyn. monkey CD122 / IL2RB protein, C-His (HEK293) [orb1516513]
>95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 43-47 kDa based on Tris-Bis PAGE result.
100 μg, 50 μgHuman IL-15RA&IL-15 [orb3002349]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 34.4&12.8 KDa. Observed: 37&17 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-15RA&IL-15 [orb3002427]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 46.9 KDa. Observed: 50-60 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgIL-15 Recombinant Protein [orb1231338]
ELISA, WB
>95% as determined by SDS-PAGE.
39.7 kDa
HEK293 cells
0.05 mg

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Protocol Information
IL-15 Recombinant Protein (orb1229848)
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