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IL-15 Recombinant Protein
Description
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The ED50 was determined by the dose - dependant stimulation of the proliferation of murine CTLL - 2 cells is 1 x 105 units/mg. |
| Target | Il15 |
| Protein Sequence | MNWIDVRYDL EKIESLIQFI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VIESGCKECE ELEERNFTEF LQSFIHIVQM FINTS |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Storage & Handling
−| Storage | The lyophilized IL-15 recombinant protein is stable for at least 2 years from date of receipt at -20˚°C. Reconstituted IL-15 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚°C. As with any protein, exposing IL-15 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL15 Protein, hFc Tag [orb1291067]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 38.9 kDa after removal of the signal peptide. The apparent molecular mass of IL15-hFc is approximately 35-55kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μgRecombinant Cyn. monkey CD122 / IL2RB protein, C-His (HEK293) [orb1516513]
>95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 43-47 kDa based on Tris-Bis PAGE result.
100 μg, 50 μgHuman IL-15RA&IL-15 [orb3002349]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 34.4&12.8 KDa. Observed: 37&17 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-15RA&IL-15 [orb3002427]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 46.9 KDa. Observed: 50-60 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgIL-15 Recombinant Protein [orb1231338]
ELISA, WB
>95% as determined by SDS-PAGE.
39.7 kDa
HEK293 cells
0.05 mg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_037261.1 |
|---|
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Protocol Information
IL-15 Recombinant Protein (orb1230638)
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