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IL23A Antibody
SKU: orb1239485
Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 8
| Tested Applications | ELISA, ICC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Equine, Guinea pig, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-IL-23 antibody (orb1239485) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23. The immunogen is located the last 50 amino acids of IL-23. |
| Target | IL23A |
| Molecular Weight | Predicted: 21kDObserved: 22 kD |
| Purification | IL-23 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | IL-23 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha
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Western Blot Validation in Human Raji Cells. Loading: 15 µg of lysates per lane. Antibodies: IL-23 orb1239485 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: Absence of blocking peptide, Lane B: Presence of blocking peptide.
Western Blot Validation in Human Cell Lines. Loading: 20 µg of lysates per lane. Antibodies: IL-23 orb1239485, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-23 recombinant protein. Antibodies: IL-23 orb1239485 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of IL-23 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23 with orb1239485 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunocytochemistry Validation of IL-23 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23 antibody (orb1239485) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of IL-23 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23 with orb1239485 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunohistochemistry Validation of IL-23 in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IL-23 antibody (orb1239485) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues (Yan et al., 2019).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL23A Antibody (orb1239485)
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