Mouse IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb347553

Description

Mouse IgG F(ab')2 antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:16,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
Reactivity	Mouse
Application Notes	Anti-Mouse IgG F(ab')2 Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Mouse IgG F(ab')2 fragment
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c) or Human Serum Proteins.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti-Mouse IgG F(ab')2 Antibody peroxidase Conjugation, Rabbit Anti-Mouse IgG Fab2 Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG Fab2 Fragment HRP Conjugated Antibody

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Mouse IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

Titers for anti-Gal antibodies. Serial 1:2 dilutions of serum from α1, 3galactosyltransferase knockout mice were incubated on plates coated with α-gal–linked bovine serum albumin. Titers of 1:160 or greater were considered immunity comparable with human titers and appropriate for use in experimentation. Highlighted area in yellow shows titers of tested mice at 1:320 and −1:640, greater than the needed threshold.

Titers for anti-Gal antibodies. Serial 1:2 dilutions of serum from α1, 3galactosyltransferase knockout mice were incubated on plates coated with α-gal-linked bovine serum albumin. Titers of 1:160 or greater were considered immunity comparable with human titers and appropriate for use in experimentation.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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