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Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed
SKU: orb347385
Description
Images & Validation
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Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:180,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Mouse IgG whole molecule |
| Purity | HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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