PCAF Rabbit mAb [KD Validated] Antibody

SKU: orb1950727

Description

Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A.

Images & Validation

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Tested Applications	FC, IP, WB
Dilution range	WB 1:500~1:2000 IP 1:50 FC 1:50
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Immunogen	A synthesized peptide derived from human KAT2B / PCAF
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer	For research use only

Alternative Names

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CAF; GCN5; GCN5L; GCN5L1; KAT2B; P/CAF; p300/CBP??P300/CBP-associated factor??Lysine acetyltransferase 2B??PCAF

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of KAT2B / PCAF expression in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.

Quick Database Links

UniProt

Q92831

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation