Phospho-Histone H3 (Ser28) Rabbit mAb

SKU: orb1564798

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Description

Research Area

Epigenetics & Chromatin

Images & Validation

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Item 1 of 3

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG
Clone No.	R03-7K5
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser28 of human Histone H3
Target	H3C1
Molecular Weight	Calculated MW: 15 kDa; Observed MW: 15 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
Concentration	0.3 mg/ml
Disclaimer	For research use only

Alternative Names

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H3 histone; family 3A; H3 histone; family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3

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Western blot analysis of Histone H3 (Phospho-Ser28) in 3T3, Hela lysates using Phospho-Histone H3 (Ser28) antibody.

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Histone H3 (Phospho-Ser28) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Histone H3 (Phospho-Ser28) (green) in Hela using Histone H3 (Phospho-Ser28) antibody, and DAPI (blue).

Quick Database Links

Gene Symbol

H3C1

UniProt

P68431

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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