PU.1 Antibody / SPI1

SKU: orb534180

Description

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). [UniProt]

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the PU.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b, kappa
Clone No.	TFPU1-1
Immunogen	A portion of amino acids 16-170 from the human protein was used as the immunogen for the PU.1 antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone TFPU1-1). Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.

IHC staining of FFPE human lymph node with PU.1 antibody (clone TFPU1-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human Hodgkin's lymphoma with PU.1 antibody (clone TFPU1-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human Ramos cells with PU.1 antibody (green, clone TFPU1-1) and Phalloidin (red).

Quick Database Links

UniProt

P17947

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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