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Rabbit HSP27 Antibody
SKU: orb1522485
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 155 and C-terminus |
| Target | HSP27 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−28 kDa heat shock protein; CMT2F; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HEL-S-102; HMN2B; HS.76067; HSP 27; Hsp25; HSP27; HSP28; HspB1; SRP27; stress-responsive protein 27
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Detection of human HSP27 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSP27 antibody orb1522485 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human HSP27 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSP27 antibody orb1522485 used for IP at 6 µg per reaction.
Detection of human HSP27 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSP27 (Cat. No. orb1522485) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001531.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit HSP27 Antibody (orb1522485)
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