Rabbit PAM Antibody

SKU: orb1527709

Description

PAM Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - Not recommended.
Reactivity	Human
Predicted Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 2475 and 2525
Target	PAM
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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E3 ubiquitin-protein ligase MYCBP2; Highwire; myc-binding protein 2; Myc-bp2; PAM; pam/highwire/rpm-1 protein; Phr; protein associated with Myc; RING-type E3 ubiquitin transferase MYCBP2

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Detection of human PAM by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PAM antibody orb1527709 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PAM was also immunoprecipitated by rabbit anti-PAM antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Quick Database Links

Gene Symbol

PAM

UniProt

O75592

RefSeq (Protein):NP_055872.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_055872.3

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Protocol Information

Western Blot (IB, immunoblot)

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