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Recombinant Human CD28
Description
Images & Validation
−
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Reactivity | Human |
| Tag | C-terminal His Tag |
| Molecular Weight | 40 kDa |
| Expression Region | 19-152 |
| Purity | Greater than 90% as determined by SDS-PAGE |
Storage & Handling
−| Storage | The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle. |
|---|---|
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4 |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human/Cynomolgus CD28 [orb2995095]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.7 KDa. Observed: 30-40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgBiotinylated Human/Cynomolyus CD28 [orb2993831]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 44.1 KDa. Observed: 60-90 KDa, reducing conditions
20 μg, 100 μgHuman/Cynomolgus CD28 [orb2994044]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 41.7 KDa. Observed: 40-75 KDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μgMouse CD28 [orb2994528]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 43 KDa. Observed: 60 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μgHuman/Cynomolgus CD28 [orb2993961]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.3 KDa. Observed: 40-85 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Protocol Information
Recombinant Human CD28 (orb2296568)
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