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Human CD40
SKU: orb2994010
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human CD40L-His-Flag(Cat#orb2993075) at 10μg/ml (100 μl/well)can bind Human CD40-Fc(Cat#orb2994010). The ED50 of Human CD40-Fc(Cat#orb2994010)is 0.45 ug/ml.. BLI: Loaded Human CD40-Fc(Cat#orb2994010) on AHC Biosensor, can bind Human CD40L-mFc(Cat#orb2993921) with an affinity constant of 0.13 nM as determined in BLI assay. (Regularly tested) Loaded Human CD40L-His(Cat#orb2994033) on HIS1K Biosensor, can bind Human CD40-Fc(Cat#orb2994010) with an affinity constant of 0.16 uM as determined in BLI assay. |
| Tag | C-Fc |
| Molecular Weight | Predicted: 46.3 KDa. Observed: 50-65 KDa, reducing conditions |
| Expression Region | Glu21-Arg193 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS,pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Tumor Necrosis Factor Receptor Superfamily member 5; B-Cell Surface Antigen CD40; Bp50; CD40L Receptor; CDw40; CD40; TNFRSF5
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Human CD40 (orb2994010)
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