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Human Siglec-10
SKU: orb2993873
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human Siglec10 mAb(Cat#orb2993121) at 2μg/ml (100 μl/well) can bind Human Siglec-10-mFc(Cat#orb2993873 ) The ED50 of Human Siglec-10-mFc(Cat#orb2993873 ) is 18.16 ng/ml.. BLI: Loaded Anti-Human Siglec-10 mAb-Fc (Cat#orb2993121) on AHC Biosensor, can bind Human Siglec-10 mFc(Cat#orb2993873) with an affinity constant of -3 nM as determined in BLI assay. |
| Tag | C-mFc |
| Molecular Weight | Predicted: 84.6 KDa. Observed: 110-120 KDa, reducing conditions |
| Expression Region | Met17Thr546 |
| Purity | SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SIGLEC10; MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7
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−Mouse Siglec-10 [orb2993793]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 85.6 KDa. Observed: 110-135 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman Siglec-10 [orb2993872]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 85.1 KDa. Observed: 110-120 KDa, reducing conditions
10 μg, 50 μg, 1 mg, 500 μgBiotinylated Human Siglec-10 [orb2993776]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 86.9 KDa. Observed: 100-130 KDa, reducing conditions
20 μg, 100 μg- Item 1 of 3
- Item 1 of 1
Human SIGLEC10 Protein, His Tag [orb689432]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 59.0 kDa after removal of the signal peptide.The apparent molecular mass of SIGLEC10-His is approximately 70-100 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μg
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Human Siglec-10 (orb2993873)
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