Recombinant Mouse PD-L1 Protein

SKU: orb2994458

Description

Recombinant Mouse Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a human IgG1 Fc tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Mouse
Biological Activity	ELISA: Immobilized Mouse PD-L1-Fc(Cat#orb2994458) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb-Fc(Cat#orb2993144).The ED50 of Anti-Human PDL1 mAb-Fc(Cat#orb2993144) is 9.72 ng/ml.
Tag	C-Fc
Molecular Weight	Predicted: 51.9 KDa. Observed: 72-90 KDa, reducing conditions
Expression Region	Phe19-Thr238
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Programmed cell death 1 ligand 1Cd274; programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; programmed death ligand 1; B7 homolog 1; B7-H1; CD274

Human PD-L1 Protein, mFc-His tag [orb689384]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Mouse CD274/PD-L1/B7-H1 Protein, C-Fc [orb3154773]
Applications:
ELISA, SDS-PAGE, WB
Purity:
>90% as determined by SDS-PAGE.
MW:
53.31 kDa
Sizes Available: 50 μg, 100 μg, 1 mg
Recombinant Mouse CD274/PD-L1/B7-H1 Protein, N-His [orb2962526]
Applications:
ELISA, SDS-PAGE, WB
Purity:
>90% as determined by SDS-PAGE.
MW:
27.19 kDa
Sizes Available: 1 mg, 50 μg, 100 μg

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Quick Database Links

UniProt

Q9EP73

UniProt Details

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No UniProt data available

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