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Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) |
| Reactivity | Human, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | S1MT-2 |
| Immunogen | Recombinant human protein was used as the immunogen for this SUMO1 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot testing of SUMO1 antibody and human HeLa lysate (clone S1MT-2).
IHC staining of human tonsil with SUMO1 antibody (clone S1MT-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.
IHC staining of rat pancreas with SUMO1 antibody (clone S1MT-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.
Immunofluorescent testing of PFA-fixed human HepG2 cells with SUMO1 antibody (green, clone S1MT-2) and Phalloidin (red).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
SUMO1 Antibody (orb750133)
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