TNF-alpha Recombinant Protein

SKU: orb1227719

Featured

Description

Research Area

Signal Transduction

Images & Validation

−

Item 1 of 1

Tested Applications	ELISA, WB
Application Notes	This recombinant protein can be used for E, WB. For research use only.

Key Properties

−

Source	HEK293 cells
Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 ug/mL (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL.
Target	TNF-alpha
Tag	His Tag
Molecular Weight	18.2 kDa
Protein Sequence	Val 77 - Leu 233
Purity	>95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method.

Storage & Handling

−

Storage	Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFalpha

Human TNFa Protein, His Tag [orb689423]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Human Ephrin-A1 Protein [orb2993974]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 85% as determined by reducing SDS-PAGE.
MW:
Predicted: 46.5 KDa. Observed: 55-60 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immobilized Human TNF-alpha, His Tag at 0.2 ug/ml (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL.

Quick Database Links

Gene Symbol

TNF-alpha

RefSeq (Protein):NP_000585.2

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000585.2

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols