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Trypsin Inhibitor Antibody
SKU: orb2652727
Description
Images & Validation
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| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution range | ELISA: 1:5,000 - 1:25,000, IP: 1:100, WB: 1:500 - 1:3,000 |
| Reactivity | Plant |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Trypsin Inhibitor [Soybean] |
| Purity | Anti-trypsin inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean]. Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
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Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody. Lane 1: Opal PreStained Molecular Weight Marker. Lane 2: Trypsin Inhibitor [50 ng]. Primary Antibody: Anti-Trypsin Inhibitor at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 mins at RT. Expect: ~25kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Trypsin Inhibitor Antibody (orb2652727)
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