Type IV Collagen Antibody

SKU: orb1251418

Description

Research Area

Cancer Biology, Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	ELISA
Reactivity	Canine, Equine, Gallus, Guinea pig, Human, Mammal, Mouse, Porcine, Primate, Rabbit, Rat, Rodent, Sheep
Application Notes	Type IV Collagen Antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, flow cytometry, western blotting, and immunoprecipitation assays.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Type IV collagen
Purification	Purified by affinity chromatography on type IV collagen covalently linked to agarose; cross-adsorbed against Collagen types I, II, III, V, and VI for minimal reactivity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	0.4 mg/mL
Disclaimer	For research use only

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Human gastric cancer tissue was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by Swine Anti-Goat IgG (H + L), Human/Rat/Mouse SP ads-HRP.

Frozen sections of E12.5 eyes from Col4a1 + / + mice were stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by a secondary antibody and DAPI.

Itga3-/-/Gpr56-/- E11.5 mouse neocortex section was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418), ant-Tuj1, and anti-Nestin followed by secondary antibodies.

Frozen human nasal mucosa explant section 24 h post infection with HSV1 was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) and anti-HSV1 gD followed by a secondary reagents.

Frozen C57BL/6 mouse quadricep muscle section was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by a secondary antibody.

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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