Type IV Collagen Antibody

SKU: orb1251418

This product can be custom made

Description

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Item 1 of 6

Tested Applications	ELISA
Reactivity	Canine, Equine, Gallus, Guinea pig, Human, Mammal, Mouse, Porcine, Primate, Rabbit, Rat, Rodent, Sheep
Application Notes	Type IV Collagen Antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, flow cytometry, western blotting, and immunoprecipitation assays.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Type IV collagen
Purification	Purified by affinity chromatography on type IV collagen covalently linked to agarose; cross-adsorbed against Collagen types I, II, III, V, and VI for minimal reactivity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	0.4 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

IHC analysis of orb1251418.

Human gastric cancer tissue was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by Swine Anti-Goat IgG (H + L), Human/Rat/Mouse SP ads-HRP.

Frozen sections of E12.5 eyes from Col4a1 + / + mice were stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by a secondary antibody and DAPI.

Itga3-/-/Gpr56-/- E11.5 mouse neocortex section was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418), ant-Tuj1, and anti-Nestin followed by secondary antibodies.

Frozen human nasal mucosa explant section 24 h post infection with HSV1 was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) and anti-HSV1 gD followed by a secondary reagents.

Frozen C57BL/6 mouse quadricep muscle section was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by a secondary antibody.

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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