Ubiquitin Antibody

SKU: orb2641382

Description

Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Images & Validation

−

Item 1 of 5

Tested Applications	ELISA, IHC-P, WB
Dilution range	ELISA (order BSA/sodium azide-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the Ubiquitin antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	UBB/2122
Immunogen	A portion of amino acids 1-119 from the human protein was used as the immunogen for the Ubiquitin antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Similar Products

−

Item 1 of 15
LC3 antibody [orb33328]
Applications:
ICC, IF, IHC-P, WB
Predicted Reactivity:
Bovine
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg
Item 1 of 4
Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody [orb312554]
Applications:
IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:
Bovine, Equine, Monkey, Porcine, Sheep
Reactivity:
Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 200 μl, 100 μl
Item 1 of 16
Ubiquitin Mouse Monoclonal Antibody [orb704536]
Applications:
ICC, IF, IHC-Fr, IHC-P
Predicted Reactivity:
Mouse, Rat
Reactivity:
Human, Mouse, Rat
Species/Host:
Mouse
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 200 μg, 100 μl, 50 μl, 200 μl
Item 1 of 12
PGP9.5 antibody [orb6713]
Applications:
ELISA, ICC, IF, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg
Item 1 of 12
SYVN1 Rabbit Polyclonal Antibody [orb11440]
Applications:
FC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:
Bovine, Canine
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl, 200 μl, 50 μl

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/2122). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human Raji cell lysate with Ubiquitin antibody (clone UBB/2122). Expected molecular weight ~9 kDa.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Ubiquitin antibody (clone UBB/2122). These results demonstrate the foremost specificity of the UBB/2122 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Ubiquitin antibody (clone UBB/2122) as confirmation of integrity and purity.

Quick Database Links

UniProt

P0CG48, P62979, P0CG47, P62987, P62988

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols