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CD3 Antibody

SKU: orb248908

Description

The CD3 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD3. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics, Immunology

Images & Validation

Tested ApplicationsFC, IF
Dilution RangeIF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
ReactivityHuman
Application Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
Clone No.OKT3
ImmunogenNative purified human CD3.
PurificationThe antibody was purified by affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceMouse IgG2a. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

T3D; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD antigen CD3d; T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD antigen CD3e; T3G; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD antigen CD3g; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

CD3 Antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody, followed by anti-mouse IgG PE.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

CD3 Antibody (orb248908)

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Available Sizes

Select a size below

30 μl
¥ 1,690.00
50 μl
¥ 2,080.00
100 μl
¥ 3,250.00
200 μl
¥ 4,550.00
DispatchUsually dispatched within 5-10 working days
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