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CD3e Antibody
SKU: orb705554
Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000, IHC-P: 1:100-1:300 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within human CD3e. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e
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![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_1.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_2.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_3.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_4.png)
![Anti-CD3 epsilon [YTH 12.5]](/images/pub/media/catalog/product/NewWebsite/35/orb411552_5.png)
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Immunohistochemical analysis of CD3e staining in human diffuse large b-cell lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of CD3e staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of CD3e staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
CD3e Antibody (orb705554)
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